My research is focused on the Haematopoietic Stem Cell to delineate the cellular and molecular pathways governing stem cell fate decisions and lineage development within the haematopoietic system. Although bone marrow or haematopoietic stem cell (HSC) transplantation is a successful cell replacement therapy, the full clinical potential of HSCs need to be developed, and require a better understanding of the normal HSC fate decisions and blood lineage development. It is important to understand the normal cellular origin and perturbed genetics of leukaemic stem cells.
Our aim is to understand the delineation of the cellular and molecular pathways governing normal HSC lineage commitment and development and also the role of positive and negative regulators of HSC self- renewal and lineage commitment with a major emphasis on positive and negative regulators of cytokine receptor signalling, the notch pathways, as well as intrinsic regulators of these processes. Using this knowledge, tools are developed to achieve ex vivo expansion of HSCs for improved transplantation results. We also focused on the identification of the leukaemic stem cells. Major efforts are devoted towards the identification, cellular and molecular characterization, as well as clinical surveillance of leukaemic stem cells. Hematopoietic stem cells are interesting target cells for gene therapy because they have an enormous proliferative capacity and can be used to repopulate the entire hematopoietic system in humans and experimental animals following bone marrow transplantation. Following effective gene transfer into hematopoietic stem cell of a patient, the genetically modified cells can be transplanted back to the patient and will be expected to produce genetically corrected cells of all blood lineages for a long time, perhaps for a lifetime.
1. Establishment of cord blood development to NK, B and T cells
2. Evaluation of B and T cell derived lymphoma mechanisms in order to use cord blood stem cell treatment property
3. Characterization of cord blood stem cell-derived NK cell
4. Characterization of acute lymphoblastic leukemia
1. Gholami, MSc student
2.Momeneh Mohamadi, PhD student
3. Amine Falahi
4.Monireh Zareh, MSc
5. Momeneh Mohamadi, PhD student
6. Anahita Mohammadi, MSc
Seyedeh Momeneh Mohammadi, Daryosh Mohammadnejad, Abbas Ali Hosseinpour Feizi, Ali Akbar Movassaghpour, Soheila Montazer Saheb, Hojjatollah Nozad Charoudeh. Inhibition of c-REL using siRNA increased apoptosis and decreased proliferation in pre-B ALL blasts: Therapeutic implications. Leukemia Research. Available online 26 August 2017.
Davoud Hasanzadeh, Majid Mahdavi, Gholamreza Dehghan, Hojjatollah Nozad Charoudeh. Farnesiferol C induces cell cycle arrest and apoptosis mediated by oxidative stress in MCF-7 cell line. Toxicology Reports. 2017 (DOI: 10.1016/j.toxrep.2017.07.010)
Aliyari Serej Z, Ebrahimi Kalan A, Mehdipour A, Nozad Charoudeh H. Regulation and roles of CD26/DPPIV in hematopoiesis and diseases. Biomed Pharmacother. 2017 Apr 24; 91:88-94. doi: 10.1016/j.biopha.2017.04.074.
Anahita Mohammadian, Elahe Naderali, Seyedeh Momeneh Mohammadi, Aliakbar Movasaghpour, Behnaz Valipour, Mohammad Nouri, Hojjatollah Nozad Charoudeh. Cord Blood Cells Responses to IL2, IL7 and IL15 Cytokines for mTOR Expression Article in Advanced Pharmaceutical Bulletin 7(1):81-85
Fallahi S, Mohammadi SM, Tayefi Nasrabadi H, Alihemmati A, Samadi N, Gholami S, Shanehbandi D, Nozad Charoudeh H. Impact of C-rel inhibition of cord blood-derived B-, T-, and NK cells. J Immunotoxicol. 2017 Dec;14(1):15-22.
Mohammadi Seyedeh Momeneh, Mohammad Nejad Daryosh, Nozad Charoudeh Hojjatollah. Genetic alterations in B-acute lymphoblastic leukemia. Acta Haematologica Polonica. Available online 6 December 2016
Balal Khalilzadeh, Nasrin Shadjou, Hadi Afsharan, Morteza Eskandani, Hojjatollah Nozad Charoudeh, Mohammad-Reza Rashidi. Nanoparticle as signal amplification element on ultra-sensitive electrochemiluminescence determination of caspase-3 activity and apoptosis using peptide based biosensor. BioImpacts, 2016, 6(3), 135-147
Brazvan B, Farahzadi R, Mohammadi SM, Montazer Saheb S, Shanehbandi D, Schmied L, Soleimani Rad J, Darabi M, Nozad Charoudeh H. Key Immune Cell Cytokines Affects the Telomere Activity of Cord Blood Cells In vitro.Adv Pharm Bull. 2016 Jun;6(2):153-61.
Sadallah S, Schmied L, Eken C, Charoudeh HN, Amicarella F, Schifferli JA. Platelet-Derived Ectosomes Reduce NK Cell Function. J Immunol. 2016 Sep 1;197(5):1663-71.
Mahmoud Izadi, Hojjatollah Nozad Charoudeh, Mohammadreza Rashidi, Morteza Milani , Mohammad Rahmati-Yamchi. Antiproliferative effects of clofarabine on AGS gastric adenocarcinoma cell line.SILVAE GENETICA online June 2015.
Zeynab Aliyari1, Nahid Khaziri3, Balal Brazvan, Manizheh Saayah Melli, Hamid Tayefi Nasr abadi, Abolfazl Akbarzadeh & Hojjatollah Nozad Charoudeh.Key immune cell cytokines have a significant role in the expansion of CD26 population of cord blood mononuclear cells. Artificial Cells, Nanomedicine, and Biotechnology, 2015; Early Online: 1–8
Zeynab Aliyari1, Forogh Alemi, Balal Brazvan, Hamid Tayefi Nasrabadi, Hojjatollah Nozad Charoudeh. CD26+ Cord Blood Mononuclear Cells Significantly Produce B, T, and NK Cells. Iran.J.Immunol.VOL.12 NO.1March2015.
Charoudeh HN, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M. Modulation of natural killer cell KIR repertoire by cytomegalovirus infection. European Journal of Immunology. 2013; 43 (2):480-487.
Charoudeh HN, Schmied L, Gonzalez A, Terszowski G, Czaja K, Schmitter K, Infanti L, Buser A, Stern M. Quantity of HLA-C surface expression and licensing of KIR2DL+ natural killer cells. Immunogenetics. 2012; 64 (10):739-745.
Charoudeh HN, Schmitter K, Buser A, Gonzalez A, Stern M. A polymorphism affecting HLA-C surface expression associates with herpes simplex virus and cytomegalovirus immunoglobulin G seropositivity Tissue Antigens. 2012; 80 (30): 263-264.
Tang Y, Peitzsch C, Charoudeh HN, Cheng M, Chaves P, Jacobsen SE, Sitnicka E. Emergence of NK-cell progenitors and functionally competent NK-cell lineage subsets in the early mouse embryo. Blood. 2012; 120 (1):63-75.
Charoudeh HN, Tang Y, Cheng M, Cilio CM, Jacobsen SE, Sitnicka E. Identification of an NK/T cell-restricted progenitor in adult bone marrow contributing to bone marrow- and thymic-dependent NK cells. Blood. 2010; 116 (2):183-192.
Cheng M1, Charoudeh HN, Brodin P, Tang Y, Lakshmikanth T, Höglund P, Jacobsen SE, Sitnicka E. Distinct and overlapping patterns of cytokine regulation of thymic and bone marrow derived NK cell development. Journal of Immunology. 2009; 182 (3):1460-1468.
Buza-Vidas N1, Cheng M, Duarte S, Charoudeh HN, Jacobsen SE, Sitnicka E. FLT3 receptor and ligand are dispensable for maintenance and post-transplantation expansion of mouse hematopoietic stem cells. Blood. 2009; 113 (15): 3453-3460.
Cheng M1, Li Y, Wu J, Nie Y, Li L, Liu X, Charoude HN, Chen H. IL-8 induces imbalances between nitric oxide and endothelin-1, and also between plasminogen activator inhibitor-1 and tissue-type plasminogen activator in cultured endothelial cells. Cytokine. 2008; 41 (1): 9-15.
Buza-Vidas N1, Cheng M, Duarte S, Charoudeh HN, Jacobsen SE, Sitnicka E. Crucial role of FLT3 ligand in immune reconstitution after bone marrow transplantation and high-dose chemotherapy. Blood. 2007; 110 (1): 424-432.